Prof. Asad U Khan Ph.D.,FRSC,FAMS,FBRSGroup Leader:
Medical Microbiology and Molecular Biology lab.
Interdiscipilinary Biotechnology Unit
Aligarh Muslim University
Prof. Asad U Khan graduated in Chemistry and did his post graduate in Biotechnology in 1994 and obtained a doctorate in Biochemistry from Aligarh Muslim University in association with International Centre for Genetic Engineering and Biotechnology, New Delhi.He joined as lecturer in 1997 and later proceeded for his post-doctoral research in RUTGERS University, New Jersey, USA during 2000-2003 for three years. Currently he is Professor and Coordinator in Biotechnology Unit. Dr. Khan is recipient of many Fellowships and awards. He is recipient of BOYSCAST Fellowship of DST, Government of India to work as Visiting Scientist in one of the laboratories in University of Napoli, Italy during 2005. In year 2010 Dr. Khan has visited Hospital Biceter , Paris under DBT-CREST award programme of Department of Biotechnology. He has received grants from DST CSIR DBT ICMR and CCRUM. Dr Khan is member of Editorial board of several International journals. He has edited Three books and author of over 234 Research articles in the peer reviewed journals with high impact factor with H-index of 34. He is member of many national and international academic bodies. On all these achievements Aligarh Muslim University conferred an Outstanding Research Award of the year to Prof Asad Ullah Khan on the day of Sir Syed day celebration in 2014. He is also recipient of most prestigious Visitors' Award (19), Shri Om Prakash Bhasin Award (19), National Bioscience Award of DBT, Government of India in 2012 . On administrative front Prof Khan served as coordinator (Head) of the Unit from 2012 to 2015. Moreover, he has also served as coordinator of DBT-BUILDER Progarmme and DST-PURSE programme of Ministry of Science and Technology, Government of India.
Prof Asad U Khan appointed as Adjunct Professor in University of Catolica San Antonio de Murcia (UCAM) Spain for the period of two years.
Prof Asad U Khan of Biotechnology, appointed as expert member of steering committee of UK Academy of Medical Sciences and Hamied Foundation UK-India AMR Programme. and Subject Expert of ANR, France to evaluate Grants on AMR call.
Prof Asad U Khan of Biotechnology , Recognised as eminent researcher in the field of Antimicrobial Resistance by Government of India and research Council UK as per the prepared ?Scoping Report on Antimicrobial Resistance? in India which was released on November 2017 during Newton?s Award function in New Delhi.
On NDM-4 Discovery NEONA Film interviewed Prof Khan for a Documentary on Antibiotic Resistance; Link:
-Selected as Featured Article :
Invited Blog for BMC Series: Khan, AU, Maryam, L and Zarrilli, R "Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health" BMC Microbiology (2017) 17:101 DOI: 10.1186/s12866-017-1012-8
-Appointed as expert member of steering committee of UK Academy of Medical Sciences and Hamied Foundation UK-India AMR Programme.
-Appointed as Adjunct Professor in University of Catolica San Antonio de Murcia (UCAM) Spain for the period of two years (2017-29)
lpl竞猜官网Our current efforts are focused on designing new inhibitors against NDM-1 and CTX-M variants. Many synthetic molecules after being screened by QSAR and structure-based virtual screening approaches from different Databases are being tested against multidrug resistant clinical strains. Different Machine learning approaches are used to design lead molecules against enzymes. Moreover, these enzymes and their mutants are being cloned from clinical multi-drug-resistant strains to express proteins/enzyme which is being studied for their interaction and hydrolysis with inhibitors and drugs respectively in vitro using various microbiological and spectroscopic approaches. Moreover, inhibitor-enzyme sites are also being mapped through Crystallography to further understand the insight of binding interactions.
lpl竞猜官网We are also studying the mechanism of multiple drug resistance in bacteria with special reference to ESBL (extended spectrum beta lactamase) and recently known enzyme, NDM-1 and its variants among E. coli and Klebsiella pneumonia isolated from Hospital setting as well as community acquired infections. The whole family of CTX-M genes was characterised by PCR amplification and sequencing. Genetic features (Tn and IS elements) associated with these resistant markers were also studied using shotgun sequencing approach and PCR amplification. Microbial typing of these isolates was performed by Pulse Field Gel Electrophoresis (PFGE) and ERIC-PCR to look for molecular epidemiology of these strains for their clonal relatedness. Moreover, Replicon typing and multilocus sequence typing was also performed to understand the clonal spread of strains of bacteria.
My group has also elucidated the mode of action of natural and synthetic molecules including, Nanoparticles against infections in general and dental caries as a specific case, which is a predominant cause of tooth decay. The main reasons of dental caries are adherence and biofilm forming properties of S. mutants. Several genes are reported to play an important role in these virulent properties. Hence, we have characterised several plant-based compounds using spectroscopic approach (NMR, GC-Mass and FTIR). Gene expression of biofilm forming genes and other virulence factors are also studying using quantitative RT-PCR. Biochemical, as well as microscopic characterization (confocal, SEM and TEM), has also been performed to understand the mode of the mechanism of inhibition of Biofilm. A proteomic approach has also been incorporated to further explore the factors involved in pathogenicity/Biofilm of S. mutants. A concept of nanoparticle-based photodynamic therapy is also in use to control biofilm infections.
lpl竞猜官网Another question, we are addressing the factors/proteins involved in the expression of resistance phenotype in the bacteria carrying various resistant markers either on chromosomal or plasmid DNA. A proteomic approach has been used to understand the protein-protein interaction. Furthermore, involvement of transcription factors to express these resistant markers has also been explored.
lpl竞猜官网 We have also identified the role of drugs on Basal Transcription Machinery with special reference to anti-cancer drugs and a hypothesis was proposed whether anticancer drugs interact transcriptional machinery via interfering with histone modulation. To prove this hypothesis in vitro histone modulation assays were performed in the presence of different anticancer drugs. Furthermore, we have confirmed inhibition of acetylation (as the key regulator) by many biochemical and immunochemical approaches using monoclonal antibodies against H3K14 (Histone 3 Lysine 14 position). Moreover, binding sites were also characterised using several bioinformatics tools, biophysical techniques.